7PPA

High resolution structure of bone morphogenetic protein receptor type II (BMPRII) extracellular domain in complex with BMP10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-13
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.91589
Spacegroup name	P 21 21 21
Unit cell lengths	44.718, 47.228, 250.085
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	125.040 - 1.480
Rwork	0.165
R-free	0.20660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sf3 2hlq
RMSD bond length	0.015
RMSD bond angle	1.871
Data reduction software	DIALS (1.14.5)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	125.040	1.510
High resolution limit [Å]	1.480	1.480
Rmerge	0.111	1.850
Rmeas	0.115	1.933
Rpim	0.032	0.554
Number of reflections	89787	4320
<I/σ(I)>	11.2	1.4
Completeness [%]	100.0	99.8
Redundancy	12.7	11.7
CC(1/2)	0.999	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	294.15	14% PEG 3350 0.19 M Ammonium citrate dibasic 0.02 M Sodium citrate tribasic dihydrate