7PP2
Complex of rice blast (Magnaporthe oryzae) effector protein AVR-Pii with the host target Exo70F2 from Rice (Oryza sativa)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-05 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.97625 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 140.316, 76.621, 67.873 |
Unit cell angles | 90.00, 107.76, 90.00 |
Refinement procedure
Resolution | 66.560 - 2.690 |
R-factor | 0.253 |
Rwork | 0.252 |
R-free | 0.27920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rl5 |
RMSD bond length | 0.002 |
RMSD bond angle | 1.187 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.810 | 2.820 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmeas | 0.156 | |
Number of reflections | 19153 | 2534 |
<I/σ(I)> | 8.4 | 1.3 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 6.7 | 6.5 |
CC(1/2) | 0.997 | 0.541 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.3 M Ammonium iodide; 30% v/v PEG3350 |