7POH
Crystal structure of ZAD-domain of Serendipity-d protein from D.melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 99.161, 99.161, 117.667 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.120 - 2.850 |
| R-factor | 0.2187 |
| Rwork | 0.218 |
| R-free | 0.24040 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.542 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 99.160 | 99.160 | 3.000 |
| High resolution limit [Å] | 2.850 | 9.010 | 2.850 |
| Rmerge | 0.089 | 0.046 | 0.853 |
| Rmeas | 0.100 | 0.053 | 0.961 |
| Rpim | 0.045 | 0.025 | 0.431 |
| Total number of observations | 57927 | ||
| Number of reflections | 12833 | 444 | 1874 |
| <I/σ(I)> | 9 | ||
| Completeness [%] | 91.3 | 86.9 | 92.9 |
| Redundancy | 4.5 | 4.2 | 4.7 |
| CC(1/2) | 0.994 | 0.990 | 0.649 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 288 | 0.1M HEPES pH 7.5, 1.3M Lithium sulfate 1.3M, 0.1M Sodium chloride |
