7PO4

Assembly intermediate of human mitochondrial ribosome large subunit (largely unfolded rRNA with MALSU1, L0R8F8 and ACP)

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	GIF Quantum LS
Detector	GATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.56
Number of Particle	1073141
Software	RELION,PHENIX,EPU,Gctf,Coot
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION