7PNP

Human Angiogenin mutant-S28A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 21 21 2
Unit cell lengths	83.861, 37.335, 40.852
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.930 - 1.800
R-factor	0.20431
Rwork	0.201
R-free	0.25424
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b1i
RMSD bond length	0.011
RMSD bond angle	1.498
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.860	1.840
High resolution limit [Å]	1.800	1.800
Number of reflections	11664	559
<I/σ(I)>	21.1
Completeness [%]	93.9
Redundancy	3.3
CC(1/2)	0.999	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	Malic acid/NaOH pH 7.0, PEG 3350