7PNP
Human Angiogenin mutant-S28A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 83.861, 37.335, 40.852 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.930 - 1.800 |
R-factor | 0.20431 |
Rwork | 0.201 |
R-free | 0.25424 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b1i |
RMSD bond length | 0.011 |
RMSD bond angle | 1.498 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.860 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 11664 | 559 |
<I/σ(I)> | 21.1 | |
Completeness [%] | 93.9 | |
Redundancy | 3.3 | |
CC(1/2) | 0.999 | 0.967 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | Malic acid/NaOH pH 7.0, PEG 3350 |