7PNJ
Human Angiogenin quardruple mutant-S28AT36AS37AS87A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 34.568, 55.052, 68.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.990 - 3.100 |
R-factor | 0.25226 |
Rwork | 0.250 |
R-free | 0.27599 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b1i |
RMSD bond length | 0.003 |
RMSD bond angle | 1.022 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.660 | 3.310 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 2632 | 452 |
<I/σ(I)> | 8.6 | |
Completeness [%] | 99.9 | |
Redundancy | 7 | |
CC(1/2) | 0.987 | 0.943 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 100 mM SODIUM CITRATE PH 5.6, 25% (w/v) PEG 4000 |