7PNJ

Human Angiogenin quardruple mutant-S28AT36AS37AS87A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 2 21
Unit cell lengths	34.568, 55.052, 68.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.990 - 3.100
R-factor	0.25226
Rwork	0.250
R-free	0.27599
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b1i
RMSD bond length	0.003
RMSD bond angle	1.022
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.660	3.310
High resolution limit [Å]	3.100	3.100
Number of reflections	2632	452
<I/σ(I)>	8.6
Completeness [%]	99.9
Redundancy	7
CC(1/2)	0.987	0.943

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	100 mM SODIUM CITRATE PH 5.6, 25% (w/v) PEG 4000