7PNC
Dark state structure of Sensory Rhodopsin II solved by serial millisecond crystallography
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2019-05-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000342 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 89.750, 131.700, 51.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.440 - 2.200 |
R-factor | 0.1841 |
Rwork | 0.183 |
R-free | 0.20090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h68 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.802 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.440 | 2.140 |
High resolution limit [Å] | 2.100 | 2.110 |
Number of reflections | 18087 | 1722 |
<I/σ(I)> | 5.56 | |
Completeness [%] | 100.0 | |
Redundancy | 242 | |
CC(1/2) | 0.991 | 0.310 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 295 | CaCl 150mM, Glycine 100mM, 38%(v/v) PEG 400, pH 7.5 |