7PMM

DEAD-box helicase DbpA in the active conformation bound to a ss/dsRNA junction and ADP/BeF3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	169.690, 169.690, 111.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.390 - 3.000
R-factor	0.1985
Rwork	0.197
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB Deposition D_1292103740
RMSD bond length	0.004
RMSD bond angle	0.766
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.18.2_3874+SVN)
Refinement software	PHENIX (1.18.2_3874+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.390	3.107
High resolution limit [Å]	3.000	3.000
Rmerge	0.121	1.043
Rmeas	0.122
Rpim	0.020	0.270
Number of reflections	33298	3268
<I/σ(I)>	24.34	3.11
Completeness [%]	99.9	99.79
Redundancy	36.15
CC(1/2)	0.998	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	293	0.2 M di-Sodium tartate, 20 %(w/v) PEG 3350