7PM7
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 2)
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK III |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | HELICAL |
Resolution (Å) | 3.5 |
Software | crYOLO,SPHIRE,RELION,PHENIX,ISOLDE,EPU,Gctf,UCSF Chimera |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |