7PLS
Cryo-EM structures of human fucosidase FucA1 reveal insight into substate recognition and catalysis.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | TFS GLACIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.49 |
Software | RELION,PHENIX,Coot |
Symmetry | D2 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |