7PLQ
Crystal structure of the PARP domain of wheat SRO1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-31 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 78.513, 34.373, 132.581 |
Unit cell angles | 90.00, 101.22, 90.00 |
Refinement procedure
Resolution | 43.350 - 2.130 |
R-factor | 0.2167 |
Rwork | 0.214 |
R-free | 0.26560 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLVE |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.350 | 2.260 |
High resolution limit [Å] | 2.130 | 2.130 |
Rmeas | 0.019 | 0.166 |
Number of reflections | 37083 | 5558 |
<I/σ(I)> | 8.69 | 1.1 |
Completeness [%] | 97.3 | 89.7 |
Redundancy | 6.97 | 6.28 |
CC(1/2) | 0.996 | 0.416 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5.8 | 293 | 0.1 MES, 0.2 M ammonium sulfate, 17% (w/v) PEG 3350 |