7PLP
Human Teneurin-4 C-rich domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.7749 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 66.343, 66.343, 66.343 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.190 - 1.400 |
| R-factor | 0.1556 |
| Rwork | 0.155 |
| R-free | 0.17050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | From unpublished anomalous experiment |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.703 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.910 | 46.910 | 1.420 |
| High resolution limit [Å] | 1.400 | 7.670 | 1.400 |
| Rmerge | 0.184 | 0.092 | |
| Rmeas | 0.186 | 0.094 | 13.184 |
| Rpim | 0.029 | 0.018 | 2.082 |
| Total number of observations | 802023 | ||
| Number of reflections | 19462 | 146 | 929 |
| <I/σ(I)> | 14 | ||
| Completeness [%] | 100.0 | 99.5 | 100 |
| Redundancy | 41.2 | 29.7 | 39.9 |
| CC(1/2) | 0.999 | 0.999 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 293 | 0.085 M HEPES pH 7.5, 1.7 v/v % PEG-400, 1.7 M (NH4)2SO4, 15 w/v % glycerol |
