7PLN

Structure of the APCbeta domain of Plasmodium vivax perforin-like protein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 1 21 1
Unit cell lengths	73.740, 42.020, 87.220
Unit cell angles	90.00, 104.95, 90.00

Refinement procedure

Resolution	63.010 - 3.150
R-factor	0.2153
Rwork	0.212
R-free	0.26940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 5OUO
RMSD bond length	0.012
RMSD bond angle	1.425
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.010	3.263
High resolution limit [Å]	3.150	3.150
Rmerge	0.194	0.567
Rmeas	0.291
Rpim	0.050	0.371
Number of reflections	9099	790
<I/σ(I)>	11.32	1.68
Completeness [%]	98.4	84.67
Redundancy	13.5	2.8
CC(1/2)	0.995	0.695

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350, 0.2 M Tri-potassium citrate