7PLE
ArsH of Paracoccus denitrificans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 112.950, 112.950, 204.410 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 97.820 - 2.600 |
R-factor | 0.3151 |
Rwork | 0.255 |
R-free | 0.27750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | custom homologous model |
RMSD bond length | 0.005 |
RMSD bond angle | 0.809 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 97.820 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rpim | 0.108 | 0.226 |
Number of reflections | 47156 | 4684 |
<I/σ(I)> | 8.2 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 2 | |
CC(1/2) | 0.340 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 298.15 | 4.77 M sodium acetate, 3% w/v 6-aminohexanoic acid, 80 mM HEPES |