7PL2
Crystal structure of choline-binding module of LytB from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979312 |
| Spacegroup name | P 6 |
| Unit cell lengths | 202.100, 202.100, 26.468 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.760 - 2.980 |
| R-factor | 0.2215 |
| Rwork | 0.219 |
| R-free | 0.27250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cnl |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.540 | 3.160 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.151 | 0.621 |
| Rpim | 0.117 | 0.467 |
| Number of reflections | 12892 | 2036 |
| <I/σ(I)> | 4.2 | 1.5 |
| Completeness [%] | 97.1 | |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | Ammonium acetate Bis-Tris propane |
