7PKW

Crystal structure of VIRB8-like OrfG central and C-terminal domains of Streptococcus thermophilus ICESt3 (Gram positive conjugative type IV secretion system).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 41 3 2
Unit cell lengths	124.920, 124.920, 124.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.170 - 1.835
R-factor	0.18477
Rwork	0.183
R-free	0.21469
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zgn 3ub1
RMSD bond length	0.016
RMSD bond angle	1.712
Data reduction software	XDS (20190315)
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP (7.0.078)
Refinement software	REFMAC (7.0.078)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.835	1.835
Rmeas	0.093
Number of reflections	29698	4676
<I/σ(I)>	48.17	4.62
Completeness [%]	99.8	99.4
Redundancy	77.4	77
CC(1/2)	1.000	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	ammonium sulfate, 1.26 M; cacodylate buffer pH 6.5, 100 mM