7PKJ
Streptococcus pyogenes apo GapN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.918401 |
Spacegroup name | P 1 |
Unit cell lengths | 97.749, 99.893, 106.182 |
Unit cell angles | 77.67, 75.19, 67.12 |
Refinement procedure
Resolution | 20.030 - 1.989 |
R-factor | 0.1942 |
Rwork | 0.192 |
R-free | 0.23190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1euh |
RMSD bond length | 0.008 |
RMSD bond angle | 1.010 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.148 | 2.170 |
High resolution limit [Å] | 1.989 | 1.989 |
Rmerge | 0.304 | 1.002 |
Rmeas | 0.373 | 1.201 |
Rpim | 0.213 | 0.655 |
Number of reflections | 153369 | 7668 |
<I/σ(I)> | 3.195 | |
Completeness [%] | 87.5 | 70.72 |
Redundancy | 2.91 | 3.23 |
CC(1/2) | 0.847 | 0.396 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 0.2 M Lithium sulfate 0.1 M Bis-Tris pH 5.5 25 % PEG 3350 |