7PIZ
The structure of phosphoglucomutase from Candida albicans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-17 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.950, 86.841, 109.920 |
Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
Resolution | 25.710 - 2.150 |
R-factor | 0.1986 |
Rwork | 0.196 |
R-free | 0.25890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | A homology model based on the orthologue protein from human (PDB 5EPC)) |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.710 | 2.200 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 65230 | 4312 |
<I/σ(I)> | 4.5 | |
Completeness [%] | 95.5 | |
Redundancy | 3.4 | |
CC(1/2) | 0.979 | 0.820 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 200 mM lithium sulphate monohydrate, 100 mM Tris-HCl pH 8.5, 30% w/v polyethylene glycol 4,000 |