7PHY
Vaccinia virus E2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.167, 90.925, 147.205 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.520 - 2.300 |
R-factor | 0.1943 |
Rwork | 0.192 |
R-free | 0.23700 |
Structure solution method | SIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 0.866 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | CRANK2 |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.170 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.074 | 2.422 |
Rmeas | 0.077 | 2.526 |
Rpim | 0.021 | 0.708 |
Number of reflections | 46847 | 2338 |
<I/σ(I)> | 19 | 0.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.2 | 12.4 |
CC(1/2) | 1.000 | 0.404 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 nL of 9.7 mg/mL VACV E2 was mixed with 90 nL of reservoir, 100 mM ADA (1:1 mix of ADA pH 6.5 and 7.0), 10% MPD, and equilibrated against an 80 uL reservoir at 20C. |