Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-13 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.7999 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.610, 54.550, 66.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.165 - 0.900 |
| Rwork | 0.115 |
| R-free | 0.13930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v0u |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.987 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.170 | 0.950 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Number of reflections | 95419 | 15086 |
| <I/σ(I)> | 13.8 | 0.99 |
| Completeness [%] | 99.0 | 97.6 |
| Redundancy | 7.2 | 6.9 |
| CC(1/2) | 1.000 | 0.371 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.1 M sodium acetate pH 4.6-5.0, 6-8% (w/v) PEG 4000, 30% (v/v) glycerol |
