Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.610, 56.010, 66.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.845 - 1.090 |
| Rwork | 0.123 |
| R-free | 0.16120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v0u |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.821 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.845 | 1.160 |
| High resolution limit [Å] | 1.090 | 1.090 |
| Number of reflections | 55866 | 8789 |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.2 | 98 |
| Redundancy | 7 | 6.2 |
| CC(1/2) | 0.999 | 0.450 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 298 | 0.1 M sodium acetate pH 4.6-5.0, 6-8% (w/v) PEG 4000, 30% (v/v) glycerol |
