7PGB

NaV_Ae1/Sp1CTD_pore-SAT09 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 43
Unit cell lengths	200.865, 200.865, 327.727
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 3.600
R-factor	0.2082
Rwork	0.207
R-free	0.23210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hk7
RMSD bond length	0.003
RMSD bond angle	0.734
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	3.730
High resolution limit [Å]	3.600	3.600
Rmerge	0.166	1.000
Number of reflections	147489	14754
<I/σ(I)>	9.3
Completeness [%]	98.5
Redundancy	7
CC(1/2)	0.998	0.130

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	12-14% PEG4000 0.1M NaCl, 0.1M MgCl2, 1 M Na acetate, pH 3.2-3.6