7PF7
Apo structure of SynFtn variant D65A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 177.140, 177.140, 177.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.639 - 1.700 |
| R-factor | 0.1829 |
| Rwork | 0.182 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 5OUW |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.712 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 53.330 | 53.330 | 1.730 |
| High resolution limit [Å] | 1.690 | 7.560 | 1.690 |
| Rmerge | 0.070 | 0.022 | 4.264 |
| Rmeas | 0.071 | 0.023 | 4.292 |
| Rpim | 0.008 | 0.003 | 0.491 |
| Total number of observations | 2119682 | 24247 | 146252 |
| Number of reflections | 27095 | 388 | 1939 |
| <I/σ(I)> | 42.4 | 167.6 | 1.5 |
| Completeness [%] | 100.0 | 98.7 | 100 |
| Redundancy | 78.2 | 62.5 | 75.4 |
| CC(1/2) | 1.000 | 1.000 | 0.779 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 2M NaCl |
