7PEN

Crystal Structure of Two-Domain Laccase mutant Y230A from Streptomyces griseoflavus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97242
Spacegroup name	P 1 21 1
Unit cell lengths	77.260, 94.980, 116.270
Unit cell angles	90.00, 91.67, 90.00

Refinement procedure

Resolution	47.100 - 1.600
R-factor	0.1365
Rwork	0.135
R-free	0.16530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lhl
RMSD bond length	0.006
RMSD bond angle	0.888
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.640
High resolution limit [Å]	1.600	1.600
Rmeas	0.100	1.130
Number of reflections	220573	16337
<I/σ(I)>	10.93
Completeness [%]	99.9	99.9
Redundancy	5.63
CC(1/2)	0.990	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	296	15 % v/v PEG Smear High, 0.15 M Ammonium acetate, 0.1 M Sodium citrate