7PCZ

Functional and structural characterization of redox sensitive superfolder green fluorescent protein and variants

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9998
Spacegroup name	P 1 21 1
Unit cell lengths	51.110, 68.920, 68.450
Unit cell angles	90.00, 104.04, 90.00

Refinement procedure

Resolution	45.350 - 1.350
R-factor	0.1735
Rwork	0.172
R-free	0.19730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7pca
RMSD bond length	0.013
RMSD bond angle	1.321
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.353	1.398
High resolution limit [Å]	1.350	1.350
Rmerge	0.045	0.378
Rpim	0.021
Number of reflections	95798	6718
<I/σ(I)>	17.67
Completeness [%]	94.8
Redundancy	5.1
CC(1/2)	0.999	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	295	10% polyethylenglycol (PEG) 6000, 30% ethanol and 100 mM natriumacetate