7PCV

Crystal structure of RBM5 RRM1-zinc finger

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-31
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.872600
Spacegroup name	C 1 2 1
Unit cell lengths	60.380, 39.880, 96.850
Unit cell angles	90.00, 96.03, 90.00

Refinement procedure

Resolution	33.220 - 2.420
R-factor	0.2613
Rwork	0.258
R-free	0.31310
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.220	33.220	2.490
High resolution limit [Å]	2.420	10.840	2.420
Rmerge	0.148	0.051	1.183
Rmeas	0.179	0.062	1.458
Total number of observations	26716
Number of reflections	8712	112	535
<I/σ(I)>	5.45	14.93	0.9
Completeness [%]	97.6	95.7	81.9
Redundancy	3.067	3.027	2.733
CC(1/2)	0.989	0.999	0.247

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1M BICINE pH 8.5, 12% PEG 6000, 10% 1M cesium chloride