7PCB

The PDZ domain of SNX27 fused with ANXA2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	53.680, 59.210, 180.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.167 - 2.000
R-factor	0.1903
Rwork	0.190
R-free	0.20470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n7d 6sak
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.167	2.050
High resolution limit [Å]	2.000	2.000
Rmeas	0.125	2.490
Number of reflections	39829	2885
<I/σ(I)>	17.7	1.16
Completeness [%]	99.6
Redundancy	13.12
CC(1/2)	0.999	0.403

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Potassium thiocyanate 0.1 M Sodium bromide 0.1 M MES 6.5 12 % v/v PEG Smear High