7PC9

The PDZ domain of SYNJ2BP complexed with the PDZ-binding motif of HTLV1-TAX1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	59.990, 99.850, 173.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.327 - 2.400
R-factor	0.2045
Rwork	0.203
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n7d 2jik
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.327	2.460
High resolution limit [Å]	2.400	2.400
Rmeas	0.316	2.430
Number of reflections	41356	2991
<I/σ(I)>	8.93	1.22
Completeness [%]	99.3
Redundancy	13.46
CC(1/2)	0.995	0.449

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES pH 7.5, 10% w/v PEG 8000, 8% v/v Ethylene glycol