7PC6
DNA-binding domain of a p53 homolog from the hydrothermal vent annelid Alvinella pompejana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999987 |
Spacegroup name | P 1 |
Unit cell lengths | 55.900, 69.985, 114.849 |
Unit cell angles | 88.07, 89.92, 81.51 |
Refinement procedure
Resolution | 46.700 - 1.920 |
R-factor | 0.185582919887 |
Rwork | 0.183 |
R-free | 0.22536 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xwr |
RMSD bond length | 0.007 |
RMSD bond angle | 0.831 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.740 | 1.950 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.056 | 0.835 |
Number of reflections | 121535 | 6032 |
<I/σ(I)> | 12.5 | 1.7 |
Completeness [%] | 92.5 | 93 |
Redundancy | 3.7 | 3.8 |
CC(1/2) | 0.998 | 0.630 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein solution: 7.2 mg/ml in 25 mM HEPES, pH 7.5, 150 mM NaCl, 0.5 mM TCEP. Crystallization buffer: 22% PEG 3350, 0.2M MgCl2, 0.1M Tris, pH 8.3. Drop size: 300 nL (150 + 150). Crystals were cryoprotected with mother liquor supplemented with 23% ethylene glycol. |