7PC1
DNA binding domain of partition protein StbA of plasmid R388
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2009-05-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 31 |
Unit cell lengths | 45.623, 45.623, 34.393 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.940 - 1.900 |
R-factor | 0.203 |
Rwork | 0.200 |
R-free | 0.22220 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.923 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHENIX (1.18_3855) |
Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.400 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.032 | 0.386 |
Number of reflections | 6294 | 897 |
<I/σ(I)> | 28.5 | |
Completeness [%] | 99.3 | |
Redundancy | 5.7 | |
CC(1/2) | 0.999 | 0.864 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M Trisodium Citrate Dihydrate pH5.6, 10% (v/v) iso-propanol and 10% (v/v) PEG 4000 |