7PBK
Vibriophage phiVC8 family A DNA polymerase (DpoZ), two conformations: thumb-exo open and thumb-exo closed
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-18 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.6926 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 120.160, 158.440, 79.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.350 - 2.800 |
R-factor | 0.1956 |
Rwork | 0.192 |
R-free | 0.25810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ktq |
RMSD bond length | 0.008 |
RMSD bond angle | 0.955 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.350 | 2.870 |
High resolution limit [Å] | 2.790 | 2.790 |
Rmerge | 0.352 | 2.617 |
Rmeas | 0.359 | 2.673 |
Rpim | 0.069 | 0.529 |
Number of reflections | 1019252 | 2621 |
<I/σ(I)> | 9.7 | 1.4 |
Completeness [%] | 99.4 | 92.7 |
Redundancy | 26.5 | 23.5 |
CC(1/2) | 0.996 | 0.413 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 300 mM ammonium citrate; 12% PEG 3350. Seeded from crystals grown at 291.15 K in 1 mM hexamine cobalt; 25% v/v isopropanol (100%); 100 mM HEPES pH 7 |