7PBG
4-ethylphenol oxidase from Gulosibacter chungangensis: native structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999869 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 151.134, 96.080, 87.636 |
Unit cell angles | 90.00, 115.65, 90.00 |
Refinement procedure
Resolution | 44.230 - 1.600 |
R-factor | 0.1822 |
Rwork | 0.181 |
R-free | 0.20660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fxd |
RMSD bond length | 0.012 |
RMSD bond angle | 1.655 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.230 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.083 | 0.642 |
Rmeas | 0.092 | 0.738 |
Rpim | 0.039 | 0.353 |
Number of reflections | 140211 | 6353 |
<I/σ(I)> | 9.8 | |
Completeness [%] | 94.5 | 86.4 |
Redundancy | 4.9 | 3.9 |
CC(1/2) | 0.996 | 0.710 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M Trizma pH 8.0, 24% PEG 6000 |