7PB9
Crystal structure of tandem WH domains of Vps25 from Odinarchaeota
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-24 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 101.228, 31.461, 59.541 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.610 - 1.800 |
| R-factor | 0.2011 |
| Rwork | 0.198 |
| R-free | 0.25840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cuq |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.013 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.610 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.120 | 2.560 |
| Number of reflections | 18405 | 18405 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.7 | |
| CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 12.5% PEG3350, 12.5% PEG1K, 12.5% MPD, 0.12 M Ethylene glycols, 0.1 M Buffer3 pH 8.5 |
