7PB4
Cenp-HIK 3-protein complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.625, 56.119, 176.704 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.370 - 2.490 |
R-factor | 0.2252 |
Rwork | 0.222 |
R-free | 0.28330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ypc |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.500 | 2.600 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.130 | 0.700 |
Number of reflections | 11771 | 11771 |
<I/σ(I)> | 8.5 | |
Completeness [%] | 99.5 | |
Redundancy | 3.7 | |
CC(1/2) | 0.980 | 0.300 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 10% PEG 8k, 100 mM imidazole pH 8 |