7PB1

Structure of the human heterotetrameric cis-prenyltransferase complex in complex with magnesium, GGPP and IsPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	H 3 2
Unit cell lengths	183.470, 183.470, 112.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.860 - 2.590
R-factor	0.2075
Rwork	0.205
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6z1n
RMSD bond length	0.004
RMSD bond angle	0.689
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.867	2.640
High resolution limit [Å]	2.570	2.570
Rmeas	0.200	2.598
Number of reflections	20716	989
<I/σ(I)>	8.61
Completeness [%]	89.7
Redundancy	6
CC(1/2)	0.995	0.139

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	292	0.07 M MES, 25% w/v PEG 400, 1.5 mM MgCl2, 4.5% v/v ethanol