7PAZ

REDUCED MUTANT P80I PSEUDOAZURIN FROM A. FAECALIS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1994-05
Detector	RIGAKU RAXIS
Spacegroup name	P 65
Unit cell lengths	50.790, 50.790, 98.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.000 - 2.000
R-factor	0.2 *
RMSD bond length	0.004 *
RMSD bond angle	0.018
Data reduction software	R-AXIS
Data scaling software	R-AXIS
Refinement software	SHELXL

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.060 *	0.110
Number of reflections	9327
<I/σ(I)>	10	3
Completeness [%]	96.0 *	90 *
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.8 *		50MM SODIUM PHOSPHATE WITH 75%, pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1	phosphate	20 (mM)
3	1	1	ammonium sulfate	42 (%sat)