7PAX

Structure of the human heterotetrameric cis-prenyltransferase complex in complex with magnesium, FsPP and IPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-06
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	H 3 2
Unit cell lengths	183.987, 183.987, 112.537
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.134 - 2.000
R-factor	0.2059
Rwork	0.205
R-free	0.23250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6z1n
RMSD bond length	0.004
RMSD bond angle	0.774
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000	2.120
High resolution limit [Å]	2.000	2.000
Rmeas	0.066	2.005
Number of reflections	47371	7888
<I/σ(I)>	22.94	1.23
Completeness [%]	96.3
Redundancy	20.9
CC(1/2)	1.000	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	292	0.1 M NaCl, 0.1 M MES, 33% w/v PEG 400