7P9W

Epstein-Barr virus encoded apoptosis regulator BHRF1 in complex with Puma BH3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 32 2 1
Unit cell lengths	62.772, 62.772, 92.592
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.247 - 2.000
R-factor	0.221447812589
Rwork	0.220
R-free	0.25688
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xpx
RMSD bond length	0.002
RMSD bond angle	0.412
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.880	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.120	0.992
Number of reflections	14768	1053
<I/σ(I)>	6.4
Completeness [%]	99.9
Redundancy	4.3
CC(1/2)	0.995	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.4 M Ammonium phosphate monobasic