7P9M
BrxU, GmrSD-family Type IV restriction enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 196.875, 68.367, 129.591 |
Unit cell angles | 90.00, 94.45, 90.00 |
Refinement procedure
Resolution | 98.140 - 2.850 |
R-factor | 0.2661 |
Rwork | 0.266 |
R-free | 0.27760 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.894 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | REFMAC (CCP4 Program Suite v7.1.015) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 98.140 | 2.960 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.044 | 1.021 |
Number of reflections | 40622 | 4514 |
<I/σ(I)> | 9.8 | |
Completeness [%] | 99.8 | 98.5 |
Redundancy | 1.9 | 2 |
CC(1/2) | 0.999 | 0.368 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M Tris-HCl (pH 7.5), 0.2 M ammonium sulphate, 20% w/v PEG3350 |