7P9K
BrxU, GmrSD-family Type IV restriction enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9781 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 214.620, 67.256, 126.547 |
| Unit cell angles | 90.00, 102.05, 90.00 |
Refinement procedure
| Resolution | 72.890 - 2.120 |
| R-factor | 0.2064 |
| Rwork | 0.204 |
| R-free | 0.25150 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.903 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (CCP4 Program Suite v7.1.015) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.890 | 2.160 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.078 | 0.899 |
| Number of reflections | 99627 | 4879 |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 99.4 | 98.6 |
| Redundancy | 2 | |
| CC(1/2) | 0.994 | 0.341 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M Tris-HCl (pH 7.5), 0.2 M ammonium sulphate, 20% w/v PEG 3350 |
