Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-16 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 0.87293 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.240, 62.618, 143.620 |
| Unit cell angles | 90.00, 118.10, 90.00 |
Refinement procedure
| Resolution | 39.220 - 1.600 |
| R-factor | 0.1649 |
| Rwork | 0.164 |
| R-free | 0.19030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eca |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.141 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 1.650 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.135 | 1.321 |
| Rmeas | 0.146 | 1.430 |
| Rpim | 0.055 | 0.543 |
| Number of reflections | 161044 | 15759 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 6.8 |
| CC(1/2) | 0.997 | 0.577 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | HEPES 0.1 M, PEG 8000 5%, ETHYLENE GLYCOL 8% |
