7P8E

Crystal structure of the Receiver domain of M. truncatula cytokinin receptor MtCRE1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9755
Spacegroup name	P 31 2 1
Unit cell lengths	60.189, 60.189, 80.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.125 - 2.500
R-factor	0.176
Rwork	0.173
R-free	0.22120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4euk
RMSD bond length	0.012
RMSD bond angle	1.125
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.16_3546)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.200	2.900
High resolution limit [Å]	2.500	2.500
Rmerge	0.097	1.028
Rmeas	0.106	1.129
Rpim	0.042	0.456
Number of reflections	3859	296
<I/σ(I)>	12.9	2
Completeness [%]	62.6	13.8
Redundancy	6.2	6
CC(1/2)	0.990	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	0.1 M Tris (base), BICINE pH 8.5 0.03 MgCl2, 0.03 CaCl2 12.5% MPD, 12.5% PEG1000, 12.5% PEG 3350