7P8D
Crystal structure of the Receiver domain of A. thaliana cytokinin receptor AtCRE1 in complex with Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 88.183, 99.202, 35.623 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.230 - 1.700 |
R-factor | 0.1953 |
Rwork | 0.194 |
R-free | 0.24560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AtCRE1-REC-K |
RMSD bond length | 0.010 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.230 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.057 | 1.340 |
Rmeas | 0.059 | 1.390 |
Number of reflections | 35267 | 5583 |
<I/σ(I)> | 22.9 | 1.56 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 12.9 | 13.2 |
CC(1/2) | 1.000 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 0.03 M MgCl2, 0.03 M CaCl2 in 0.1 M MES/Imidazole at pH 6.5 and 12.5% of each PEG1000, PEG3350 and 27.5% MPD |