7P81
Crystal structure of ClpP from Bacillus subtilis in complex with ADEP2 (compact state)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 112.979, 173.075, 160.001 |
| Unit cell angles | 90.00, 91.66, 90.00 |
Refinement procedure
| Resolution | 45.510 - 2.790 |
| R-factor | 0.2356 |
| Rwork | 0.235 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ktg |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Number of reflections | 146652 | 11129 |
| <I/σ(I)> | 19.79 | |
| Completeness [%] | 96.0 | |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 100 mM sodium citrate pH 5.6, 100 mM Li2SO4 and 9~11% (w/v) PEG 6,000 |
