7P80
Crystal structure of ClpP from Bacillus subtilis in complex with ADEP2 (compressed state)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-11 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97940 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.617, 172.087, 84.137 |
Unit cell angles | 90.00, 118.39, 90.00 |
Refinement procedure
Resolution | 29.590 - 2.980 |
R-factor | 0.2332 |
Rwork | 0.229 |
R-free | 0.27920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tt6 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.089 |
High resolution limit [Å] | 2.980 | 2.982 |
Number of reflections | 26196 | 2324 |
<I/σ(I)> | 23.8 | |
Completeness [%] | 96.1 | |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295 | 100 mM sodium acetate pH 4.6, 500 mM potassium thiocyanate |