7P6M
Hydrogenated refolded hen egg-white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.700001 |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.120, 30.700, 33.540 |
| Unit cell angles | 88.97, 72.77, 69.50 |
Refinement procedure
| Resolution | 22.460 - 0.890 |
| Rwork | 0.125 |
| R-free | 0.15310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 7ave |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.460 | 0.920 |
| High resolution limit [Å] | 0.890 | 0.890 |
| Rmerge | 0.083 | 0.647 |
| Rmeas | 0.098 | 0.762 |
| Rpim | 0.052 | 0.398 |
| Number of reflections | 68446 | 6717 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 96.2 | 94.3 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.996 | 0.799 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.5 | 291 | 0.05 M sodium acetate pH 4.5, 0.3 M sodium nitrate, micro seeds of G. gallus HEWL |
