7P6G

Crystal structure of the endoglucanase RBcel1 E135Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9798
Spacegroup name	P 21 21 21
Unit cell lengths	46.380, 99.520, 149.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.570 - 1.490
R-factor	0.1751
Rwork	0.174
R-free	0.19330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ee9
Data reduction software	XDS (20200417)
Data scaling software	XSCALE (20200417)
Phasing software	PHASER (2.7.15)
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	15.570	15.570	1.530
High resolution limit [Å]	1.490	6.670	1.490
Rmerge	0.043	0.020	1.109
Rmeas	0.046	0.022	1.202
Total number of observations	800713
Number of reflections	112931	1360	7716
<I/σ(I)>	22.27	58.57	1.69
Completeness [%]	99.4	94.6	93.1
Redundancy	7.09	5.963	6.444
CC(1/2)	1.000	0.999	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Reservoir: 0.1M Tris, 17.5% PEG600, pH8. Protein: 14 mg/ml in 20 mM sodium phosphate buffer pH 6.5. Drop: 2 ul reservoir + 2 ul protein