7P6F
1.93 A resolution X-ray crystal structure of the transcriptional regulator SrnR from Streptomyces griseus
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-30 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97622 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 113.358, 113.358, 124.943 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 51.670 - 1.930 |
Rwork | 0.178 |
R-free | 0.21670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f6v |
RMSD bond length | 0.012 |
RMSD bond angle | 1.756 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 124.940 | 1.980 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.126 | 1.670 |
Rmeas | 0.138 | 1.827 |
Rpim | 0.041 | 0.541 |
Number of reflections | 36223 | 2377 |
<I/σ(I)> | 11.6 | 1.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.4 | 11.7 |
CC(1/2) | 0.999 | 0.633 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 8.5 | 293 | 1 uL of SrnR 12.5 mg mL-1 (in 20 mM TrisHCl PH 7.5, 150 mM NaCl, 1 mM TCEP) were added to 20 uL of volatile oil, immediately followed by 1 uL of precipitant containing 0.2 M calcium acetate hydrate, 0.1 M TrisHCl 8.5, 15 % w/v PEG 4000 (G6 condition of CSSII screening by Molecular Dimensions). |