7P53

Crystal Structure of Human gamma-D-crystallin mutant C110M at 1.57 Angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-31
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979180
Spacegroup name	P 21 21 21
Unit cell lengths	33.699, 52.844, 89.554
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.510 - 1.570
R-factor	0.2175
Rwork	0.215
R-free	0.27380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hk0
RMSD bond length	0.010
RMSD bond angle	1.067
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHENIX (1.15.2_3472)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.840	1.600
High resolution limit [Å]	1.570	1.570
Rmerge	0.188	1.941
Rmeas	0.206	2.141
Rpim	0.083	0.883
Number of reflections	22817	953
<I/σ(I)>	6	0.5
Completeness [%]	99.2	85.8
Redundancy	6.1	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7	277	Crystals were obtained in 0.1M Sodium Phosphate Buffer pH 7 inducing LLPS at approximately 263K for 3hours from 150mg/mL solutions. Crystals were observed after few hours of incubation at 277K.