7P4V
GlnK1 from Methanothermococcus thermolithotrophicus with dADP at a resolution of 1.94 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-17 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.00003 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 87.296, 87.296, 46.010 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.300 - 1.940 |
R-factor | 0.198 |
Rwork | 0.197 |
R-free | 0.22570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j9d |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.601 | 2.148 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.053 | 1.711 |
Rmeas | 0.055 | 1.759 |
Rpim | 0.013 | 0.403 |
Number of reflections | 10108 | 502 |
<I/σ(I)> | 27.3 | 1.8 |
Completeness [%] | 89.1 | 60.2 |
Redundancy | 19.3 | 18.6 |
CC(1/2) | 0.999 | 0.773 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291.15 | GlnK1 crystallized at a concentration of 33 mg/ml in 25 mM TrisHCl pH 7.6, 10% glycerol, 2mM dithiothreitol and 500mM NaCl . Drop of 0.6 ul of protein was mixed with 0.6 ul of the crystallization solution in a 96-Well MRC 2-Drop Crystallization Plates in polystyrene (SWISSCI). The reservoir contained 90 ul of the following crystallization solution: 35 % w/v Pentaerythritol propoxylate (17/8 PO/OH), 100 mM MES pH 6.5, 200 mM Ammonium sulfate. |